منابع مشابه
The Isoelectric Point of Adsorbed Hemo- Globin*
It is well established that various proteins, as gelatin and albumin, can be adsorbed on various adsorbents so that the proteincoated particle of adsorbent behaves electrophoretically as a particle of protein (Loeb, 1923; Freundlich and Abramson, 1928). Dummett and Bowden (1933) have recently reported, however, that the behavior of adsorbed hemoglobin varies with the adsorbent surface. Thus, wh...
متن کاملIsoelectric Point Separations of Peptides and Proteins
The separation of ampholytic components according to isoelectric point has played an important role in isolating, reducing complexity and improving peptide and protein detection. This brief review outlines the basics of isoelectric focusing, including a summary of the historical achievements and considerations in experimental design. Derivative methodologies of isoelectric focusing are also dis...
متن کاملPIP-DB: the Protein Isoelectric Point database
UNLABELLED A protein's isoelectric point or pI corresponds to the solution pH at which its net surface charge is zero. Since the early days of solution biochemistry, the pI has been recorded and reported, and thus literature reports of pI abound. The Protein Isoelectric Point database (PIP-DB) has collected and collated these data to provide an increasingly comprehensive database for comparison...
متن کاملProteome-pI: proteome isoelectric point database
Proteome-pI is an online database containing information about predicted isoelectric points for 5029 proteomes calculated using 18 methods. The isoelectric point, the pH at which a particular molecule carries no net electrical charge, is an important parameter for many analytical biochemistry and proteomics techniques, especially for 2D gel electrophoresis (2D-PAGE), capillary isoelectric focus...
متن کاملAn algorithm for isoelectric point estimation
One of the most common techniques for separating mixtures of proteins is the two-dimensional polyacrylamide gel. In these gels, proteins are separated in one dimension based on their electromigration speeds through the gel (roughly determined by their molecular weights) and in another dimension by the pH at which their side chains cumulatively amount to a neutral charge, called the isoelectric ...
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ژورنال
عنوان ژورنال: Biology Direct
سال: 2016
ISSN: 1745-6150
DOI: 10.1186/s13062-016-0159-9